Sep 17, 2018 the main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. Technologies can be generally classified as in situ or ex situ. Ex situ conservation literally means, offsite conservation. When compared to standard practices, effluent volumes generated by bioremediation are substantially smaller reducing the problem of sludge disposal. Ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. A major advantage of ex situ bioremediation processes is that most of the decontaminated soil can be reused. Dec 29, 2011 types of in situ bioremediation1 intrinsic in situ bioremediation. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful,and obtain important design criteria. April 2006 keywords bioremediation, biodegradation, microorganism, insitu. Ex situ bioremediation ex situ bioremediation involves removal of waste materials and their collection at the place to facilitate microbial degradation. Ex situ bioremediation of soil contaminated with crude oil by use of actinomycetes consortia for process bioaugmentation 1ahmed a. For each one the principle of operation and recent. Dec 10, 2017 in situ and ex situ bioremediation technologies p2 duration. Bioremediation is a process used to treat contaminated media, including water, soil and subsurface material, by altering environmental conditions to stimulate growth of microorganisms and degrade the target pollutants.
Bioremediation can either be carried out ex situ or in situ, depending on several factors, which include but not limited to cost, site characteristics, type and concentration of pollutants. Ex situ bioremediation technologies such as biopile, bioreactors, composting and. On the other hand, the excavation features of ex situ bioremediation tend to disrupt soil structure. Introduction to in situ bioremediation of groundwater. Bioremediation process types of bioremediation examples. In situ bioremediation is the application of biological treatment to the cleanup of hazardous chemicals present in the subsurface. In situ techniques are generally less expensive, generate less dust and debris, and release less contaminants than ex situ techniques. The optimization and control of microbial transformations of organic contaminants require the integration of many scientific and engineering disciplines. These techniques involve excavating pollutants from polluted sites and subsequently transporting them to.
Each of those substances is then separated and properly disposed of. In situ and ex situ biodegradation technologies for. Biological techniques of remediation in the soil and groundwater zone 37. Performance criteria, which also determine the choice of ex situ bioremediation techniques, have been described philp and atlas 2005. A critical analysis of exsitu bioremediation technologies. Difference between in situ and ex situ bioremediation.
Ex situ bioremediation can use bioreactors and added nutrients. Ex situ bioremediation method for the treatment of groundwater. In situ bioremediation of groundwater has become one of the most widely used technologies for contaminated site treatment because of its relatively low cost, adaptability to site specific conditions, and efficacy when properly implemented. Furthermore, in situ methods are less expensive and less manageable while the ex situ methods are expensive and manageable. Bioremediation is the use of any organism metabolism to remove pollutants. The design process commonly includes bench and pilotscale treatability studies. Pdf a critical analysis of exsitu bioremediation technologies of.
Us epa engineering issue in situ and ex situ biodegradation. However, they require excavation and treatment of the contaminated soil before and, sometimes, after the actual bioremediation step. Pdf bioremediation techniquesclassification based on. Insitu soil and groundwater bioremediation techniques and. Bioremediation techniquesclassification based on site of. Mahdi 1biology department, college of science, university of basrah, basrah, iraq.
Generally, ex situ techniques apparently are more expensive compared to in situ techniques as a result of additional cost attributable to excavation. Laitinen, jarno insitu soil and groundwater bioremediation techniques and applications. Investigators cannot fully understand the details of whether and how bioremediation is occurring at a site. Icscs, 2006, manual for biological remediation techniques, international centre for soil and contaminated. Depending on the state of the contaminant to be removed, ex situ bioremediation is classified as. Ex situ bioremediation approaches include use of bioreactors, landfarming, and biopiles. Ex situ bioremediation techniques involve the excavation or removal of contaminated soil from ground. This technique is usually faster, easier to control and used to treat a wider range soil types and contaminants than in situ techniques. Ex situ processes involve the removal of the contaminated media to a treatment area. Pdf bioremediation techniquesclassification based on site.
Types of in situ bioremediation1 intrinsic in situ bioremediation. Bioremediation is a biotechnical process, which abates or cleans up contamination. Ex situ bioremediation techniques are usually considered based on. Bioremediation technologies can be generally classified as in situ or ex situ. Some sources and pathways of soil contamination are discussed. It is a type of waste management technique which involves the use of organisms to remove or utilize the pollutants from a polluted area. Mar 03, 2017 key difference in situ vs ex situ bioremediation bioremediation is a term used in biotechnology to refer the process of cleaning the polluted areas using biological organisms such as microorganisms and plants.
Ex situ bioremediation of soil contaminated with crude oil by. Ex situ bioremediation, in which contaminated soil is excavated and treated elsewhere, is an alternative. Bioremediation technologies for soil composting addition of moisture and nutrients, regular mixing for aeration biopiles ex situ aeration of soil bioventing in situ aeration of soil land treatment application of organic materials to natural soils followed by irrigation and tilling. Insitu and exsitu bioremediation technologies p1 duration. In situ bioremediation an overview sciencedirect topics. Bioremediation technologies for soil composting addition of moisture and nutrients, regular mixing for aeration biopiles exsitu aeration of soil bioventing insitu aeration of soil land treatment application of organic materials to natural soils followed by irrigation and tilling. Project manager for an in situ bioremediation project in des moines, ia. In situ and ex situ bioremediation technologies p2 youtube. It also suffers from costs associated with solid handling process e. Trick to learn in situ on site and ex situ off site conservation duration.
This technique has more disadvantages than advantages. A systematic approach to in situ bioremediation in groundwater, including decision trees on in situ bioremediation for nitrates, carbon tetrachloride, and perchlorate isb8 aug02 provides guidance for the systematic characterization, evaluation, and appropriate design and testing of isb for any biotreatable contaminant. Bioremediation techniques used in environmental biotechnology. In general, the techniques are subdivided into exsitu and insitu remediation. Ex situ bioremediation environmental biotechnology. In situ and ex situ bioremediation technologies p2 duration. The goal in evaluating in situ bioremediation is to assess whether the weight of evidence from tests such as those described above documents a convincing case for successful bioremediation. Ex situ bioremediation involves extraction of contaminated media to other location for treatment.
The soil can, however, be used for landscaping purposes. The main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. Ex situ bioremediation list of high impact articles ppts. And, ex situ bioremediation means you would scoop up the contaminated soil or pump up the contaminated water and take it somewhere else for bioremediation. It consists of placing the excavated materials into an above ground enclosure. Insitu and exsitu bioremediation technologies p1 youtube. Ex situ bioremediation technology includes most of disadvantages and limitations. Introduction to in situ bioremediation of groundwater es3. Slurry phase bioremediation slurryphase bioremediation is a biological treatment of soil taken place in aqueous medium. A systematic approach to in situ bioremediation in groundwater, including decision trees on in situ bioremediation for nitrates, carbon tetrachloride, and perchlorate isb8 aug02 provides guidance for the systematic characterization, evaluation, and appropriate.
In situ bioremediation involves treating the contaminated material at the site, while ex situ involves the removal of the contaminated material to be treated elsewhere. Some examples of bioremediation technologies are bioaugmentation, biostimulation, bioreactors, land. Bioremediation that can occur on its ownfactors effecting rate of intrinsicbioremediation. Ex situ bioremediation technologies such as biopile, bioreactors, composting and landfarming have been successfully used in the treatment of. In aerobic bioventing, air is typically injected into the subsurface to facilitate aerobic metabolism of hydrocarbons. Ex situ bioremediation requires excavation or pumping of contaminated soil or groundwater, respectively, before treatment is initiated. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. In this the contaminated soil is excavated and removed from the site as completely as possible. This technique applies a vacuum to extract soil vapor, water, and free product from the subsurface. Due to the ex situ techniques used to decontaminate the soil, much of the soil cannot be used as filling or agricultural material. Manual for biological remediation techniques umweltbundesamt.
Ex situ bioremediation techniques are unlikely to be used in some sites such as under buildings, inner city and working sites philp and atlas 2005. Sep 16, 2016 ex situ bioremediation techniques are usually considered based on. The project attained no further action after 30 months of operation of a combined in situ bioremediation and fixed. Trick to learn in situ on site and ex situ off site conservation. Solidphase systems include compost heaps, landfarming soiltreatment units and engineered biopiles. Ex situ bioremediation techniques are usually aerobic and involve treatment of contaminated soils or sediments using solid or slurryphase systems. Ex situ bioremediation processes involve an excavation of contaminated soil or pumping of groundwater before and sometimes, after the actual bioremediation treatment. On the basis of phases of contaminated materials ex situ bioremediation is classified in to two. Some examples of bioremediation technologies are bioaugmentation, biostimulation, bioreactors, landbased treatments, and fungal remediation. It was revealed that available techniques for soil decontamination can be divided in two parts, depending on where the action have place. A general essay on bioremediation of contaminated soil msu. Dec 10, 2017 in situ and ex situ bioremediation technologies p1 duration.
There are several remedies where contaminated water or solid is purified by chemical treatment, incineration, and burial in a. In situ and ex situ bioremediation bioremediation techniques are destruction techniques to stimulate the growth of microorganisms, using the contaminants as a food and energy source. Bioremediation techniques are designed to break down contaminants via the stimulation of microorganisms which use the contamination as an energy source for development and growth. The use of organisms to reduce and transform contaminants into nontoxic substances is an environmentally friendly process which does not ne. Ex situ bioremediation describes a process where contaminated soil or water is removed from the environment by biological organisms. In the use of a bioreactor, contaminated soil is mixed with water and nutrients and the mixture is agitated by a mechanical bioreactor to stimulate. Bioremediation techniques are more economical than traditional methods and pollutants can be treated on site, thus reducing exposure risks for personnel. In many cases, bioremediation is less expensive and more sustainable than other remediation alternatives. It is the process of protecting an endangered species, variety or breed, of plant or animal outside its natural habitat. Aug 03, 2016 ex situ bioremediation ex situ bioremediation involves removal of waste materials and their collection at the place to facilitate microbial degradation. Environmental protection agency, office of solid waste and emergency response, washington, d. There is more certainty about the uniformity of treatment because of the ability to homogenize, screen, and continuously mix the soil. In situ bioremediation of groundwater has become one of the most widely used technologies for contaminated site treatment because of its relatively low cost, adaptability to site specific.